45.6.TG1.7

90110506

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: mouse blood
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblast
• Produkte: Immunoglobulin G (IgG2b) ( kappa)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse BALB/c myeloma

Beschreibung der Zelllinie

Derived from a female BALB/c myeloma tumour MPC-11. The parental cell line has mutated during growth in 6-thioguanine to establish the drug resistant line 45.6.TG1.7. This line is HPRT negative and produces mouse IgG2b kappa immunoglobulins.

Anwendung

Fusion partner, immunoglobulin production

Nährmedium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 4-5x100,000 cells/ml; 5% CO₂; 37°C. Cells achieve a maximum cell density of 1-1.5x1,000,000 cells/ml.

Sonstige Hinweise

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