BW5147.G.1.4.OUA/R.1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: mouse blood
• Verpackung: tube of 5 μg 85082302-DNA-5UG; pkg of vial of cells 85082302-1VL
• Wachstumsmodus: Suspension
• Morphologie: Lymphoblast
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Allgemeine Beschreibung

For complete product information, please see BW5147.G.1.4.OUA/R.1

Ursprung der Zelllinie

Mouse AKR/J T cell lymphoma

Beschreibung der Zelllinie

This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.

Anwendung

Fusion partner

Nährmedium

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 1-6x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.