N1-S1

90011902, rat liver, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: N1-S1, 90011902
• Biologische Quelle: rat liver
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat hepatoma

Beschreibung der Zelllinie

Established from the ascitic fluid of a Novikoff hepatoma. The cells can be synchronised for cell cycle studies. N1-S1 has also been used for studies of pyrimidine metabolism, DNA synthesis and methylation and ribosomal RNA maturation.

Anwendung

Cell cycle, metabolic and mutation studies

Nährmedium

Swimm′s S-77 + 2mM Glutamine + 0.1% Pluronic F68 +10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 4x10,000 to 1,000,000 cells/ml; 5% CO₂; 37°C. Subculture every 3-4 days by centrifugation or medium addition.

Sonstige Hinweise

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