CAR

89072611, fish fin, Fibroblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: CAR, 89072611
• Biologische Quelle: fish fin
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Goldfish fin tissue

Beschreibung der Zelllinie

The cells support the growth of grunt fin agent with CPE at 20°C and 25°C. Also susceptible to infectious pancreatic necrosis virus of trout at 28°C.

Anwendung

Growth factors and virus studies: Infectious pancreatic necrotic virus

Nährmedium

BME (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin; 5% CO₂; 25°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media ch

Sonstige Hinweise

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