HL60 Ast.4

88120802, human blood, Promyeloid, blast/promyelocyte

• UNSPSC-Code: 41106514

Eigenschaften

• product name: HL60 Ast.4, 88120802
• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: 2n = 48, hyperdiploid
• Morphologie: Promyeloid, blast/promyelocyte
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian promyelocytic leukaemia

Beschreibung der Zelllinie

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.

Anwendung

Differentiation studies

Nährmedium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic.

Sonstige Hinweise

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