ML-1

88113007

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human blood
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblastoid
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human acute myeloblastic leukaemia

Beschreibung der Zelllinie

ML-1 is one of three celll lines isolated in 1978 from the peripheral blood of a 24 year old male patient with acute myeloblastic leukaemia. The cells can convert to more mature cells by the use of DMSO. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Anwendung

ML-1 has been used to compare the cell response to mafosfamide cyclohexylamine salt, 4-hydro-peroxy-cyclophosphamide and β-D-glucose-isophosphoramide mustard, which are oxazaphosphorine agents.

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9,12
D16S539: 9,12
D5S818: 12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,10
vWA: 16

Nährmedium

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442)

Subkultur-Routine

Maintain cultures between 2-9 x100,000 cells/ml; 5% CO₂; 37°C. Freeze in 10% DMSO + 90% FBS. Immediately after resuscitation, pellet cells by centrifugation at 150 x g for 5 minutes and resuspend the cell pellet in fresh medium. This is to remove the presence of DMSO which may cause differentiation of the cells if allowed to remain.

Sonstige Hinweise

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