NCTC clone 929

88102702, mouse connective tissue, Fibroblast

• Synonym(e): Earles′s Cells, L Cells, L-929 Cells, NCTC 929 Cells, NCTC-929L Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: NCTC clone 929, 88102702
• Biologische Quelle: mouse connective tissue
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 40
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse C3H/An connective tissue

Beschreibung der Zelllinie

From parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus.

Anwendung

Replication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Freeze in 10% glycerol/90% FBS. After resuscitation cells may take up to 4 days to establish growth.

Sonstige Hinweise

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