KG-1

86111306, human bone marrow, Myeloblast-like

• Synonym(e): KG1 Cells
• UNSPSC-Code: 41106514

Eigenschaften

• product name: KG-1, 86111306
• Biologische Quelle: human bone marrow
• Wachstumsmodus: Suspension
• Karyotyp: 2n = 46, diploid
• Morphologie: Myeloblast-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian bone marrow myelogenous leukaemia

Beschreibung der Zelllinie

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

Anwendung

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

Nährmedium

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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