CRFK (BVD Ag negative)

86093002, feline kidney, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: CRFK (BVD Ag negative), 86093002
• Biologische Quelle: feline kidney
• Wachstumsmodus: Adherent
• Karyotyp: Hypodiploid, modal no 36
• Morphologie: Epithelial
• Produkte: Carcinoembryonic antigen (CEA)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Cat kidney

Beschreibung der Zelllinie

CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.

Anwendung

Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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