Hep2 (HeLa derivative) Cell Line human

86030501, human cervix, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: Hep2 (HeLa derivative) Cell Line human, 86030501
• Biologische Quelle: human cervix
• Beschreibung: Human Black, cervix carcinoma
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 14
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human cervix carcinoma

Beschreibung der Zelllinie

Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Anwendung

Human Hep2 (HeLa derivative) cell line has been used for virus and tumorigenicity studies.

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. After thawing the first subculture may take 7-10 days.

Sonstige Hinweise

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