WEHI-231

85022107, mouse blood, Lymphoblast

• UNSPSC-Code: 41106514

Eigenschaften

• product name: WEHI-231, 85022107
• Biologische Quelle: mouse blood
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblast
• Produkte: Surface Immunoglobulin M (IgM)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse B cell lymphoma

Beschreibung der Zelllinie

Expresses surface IgM but does not secrete Ig. Secretion of IgM can be induced with LPS. WEHI 231 is a B cell lymphoma of BALB/c x NXB F1 origin induced by mineral oil injection.

Anwendung

Tumourigenicity studies

Nährmedium

DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-9x100,000 cells/ml; 5% CO₂; 37°C.

Sonstige Hinweise

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