S1814.PB5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human embryo
• Verpackung: tube of 5 μg 85011402-DNA-5UG; pkg of vial of cells 85011402-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Diploid
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human embryo fibroblast

Beschreibung der Zelllinie

Human embryo fibroblast cell line.

DNA-Profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9
TPOX: 9,11
vWA: 15

Nährmedium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells should also grow in DMEM or RPMI 1640. This cell line also grow well in HAT.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.