h1RPE7

from human eye, 09061602

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: human eye
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Polygonal
• Produkte: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice

Beschreibung

Ursprung der Zelllinie

An immortalized retinal pigment epithelial cell line

Beschreibung der Zelllinie

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

DNA-Profil

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19

Nährmedium

Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 24hrs.

Sonstige Hinweise

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Haftungsausschluss

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.