N1E-115-1

8062511, mouse nerve, neuronal

• UNSPSC-Code: 41106514

Eigenschaften

• product name: N1E-115-1, 08062511
• Biologische Quelle: mouse nerve
• Wachstumsmodus: Adherent
• Karyotyp: modal no. of chromosomes is 192 see reference
• Morphologie: neuronal
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse neuroblastoma

Beschreibung der Zelllinie

A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.

Anwendung

can be used as a neuronal model

Nährmedium

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Population doubling approximately 20 hours.

Sonstige Hinweise

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