C22 (Clara)

7021401, mouse lung, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: C22 (Clara), 07021401
• Biologische Quelle: mouse lung
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse lung epithelial progenitor cell

Beschreibung der Zelllinie

A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

Nährmedium

DMEM + 2 mM Glutamine + 4% Fetal Bovine Serum (FBS) + 8 supplements:

0.25 μg/ml Endothelin-1
0.01 μg/ml Interferon gamma
10 μg/ml Insulin
5 μg/ml Transferrin
7.5 μg/ml Endothelial Cell Growth Supplement (ECGS)
0.025 μg/ml Epidermal Growth Factor
0.36 μg/ml Hydrocortisone
0.02 μg/ml T3

For guidance on the supplements and preparation of the culture medium, ECACC can supply an information sheet on request - contact ECACC Technical Support.

Subkultur-Routine

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO₂; 33°C. Doubling time 48h

Sonstige Hinweise

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