BF-2

21713, fish caudal trunk, Fibroblast-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: BF-2, 00021713
• Biologische Quelle: fish caudal trunk
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Fish bluegill fry

Beschreibung der Zelllinie

The cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings (Bluegill fry, Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including viral haemorrhagic septicaemia virus (VHSV), infectious hematopoietic necrosis virus (IHNV), spring viraemia of carp virus (SVCV) and pike fry rhabdovirus (PFRV).

Anwendung

Fish virus studies

Nährmedium

EMEM(HBSS) (M5775) or EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 850 mg/L Sodium Bicarbonate(NaHCO₃) (S8761) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120 mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442); a mixture of MEM (HBSS) and MEM (EBSS) with 1-2% FBS is best for stability of monolayer and maintenance medium

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; cells replicate at 15 - 33°C, optimal temperature is 23 - 26°C; monolayer is stable for at least 10 days in maintenance medium (at 15 or 16°C for about 21 days). Fish c

Sonstige Hinweise

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