Direkt zum Inhalt
Merck

C-39438

Sigma-Aldrich

Adipocyte Nutrition Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

Synonym(e):

Recommended with C-27431

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352207
NACRES:
NA.71

Form

aqueous solution

Verpackung

pkg of 1 vial

Methode(n)

cell culture | mammalian: suitable

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

Adipocyte Nutrition Medium SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-27431

Anwendung

Adipocyte Nutrition Medium can be used to cultivate mature adipocytes. The medium is optimized for primary human cells but can also be used for murine adipocytes.Recommended for:Human Adipocytes

Komponenten

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Qualität

All lots of PromoCell Preadipocyte/Adipocyte Media are subjected to comprehensive quality control tests using primary human white preadipocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested preadipocytes. In addition, the Preadipocyte Differentiation Medium/Adipocyte Nutrition Medium are tested for the capacity to induce the terminal differentiation of preadipocytes into mature adipocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Angaben zur Herstellung

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.03 ml / mld-Biotin: 8 µg / mlInsulin (recombinant human): 0.5 µg / mlDexamethasone: 400 ng / ml

Lagerung und Haltbarkeit

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subkultur-Routine

Click here for more information.

Empfehlung

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Artikel

Primary human white preadipocytes and culture media for the expansion of undifferentiated preadipocytes, differentiation to adipocytes, and cultivation of mature adipocytes. Protocols for handling, cell passaging, media, and product use.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.