Direkt zum Inhalt
Merck
Alle Fotos(1)

Wichtige Dokumente

Gesamtdokumentation anzeigen

C2780

Sigma-Aldrich

Chondroitinase AC aus Flavobacterium heparinum

lyophilized powder, 0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

Synonym(e):

Chondroitin-AC-Lyase

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

CAS-Nummer:
9047-57-8
EC-Nummer:
4.2.2.5 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00130796
UNSPSC-Code:
12352204
NACRES:
NA.54

Konjugat

(Glucosaminoglycan)

Qualitätsniveau

200

Form

lyophilized powder

Spezifische Aktivität

0.5-1.5 units/mg protein (using chondroitin sulfate A as substrate)

Zusammensetzung

Protein, ~15% Lowry

Löslichkeit

0.02 M phosphate buffer: soluble (pH 7.0)

Fremdaktivität

Glycosaminoglycan (GAG) degradation enzymes, may contain trace amount

Lagertemp.

−20°C

Suchen Sie nach ähnlichen Produkten? Aufrufen Leitfaden zum Produktvergleich

Verwandte Kategorien

Anwendung

Chondroitinase AC from Sigma has been used for the large scale preparation of glycosaminoglycan (GAG) fractions during the study of structural and sequence motifs in dermatan sulfate.
Chondroitinase AC has been applied to the analysis of chondroitin sulfate in commercial samples such as dietary supplements.

Biochem./physiol. Wirkung

Chondroitinase AC from Flavobacterium heparinum is an enzyme that cleaves sulfated and non-sulfated polysaccharide chains with (1-4) linkages between hexosamines and glucuronic acid residues, by an elimination mechanism. The resulting oligosaccharide products are mainly disaccharides with unsaturated uronic acids. Chondroitinase AC specifically degrades chondroitin sulfates A and C, but not chondroitin sulfate B (dermatan sulfate).

Sonstige Hinweise

Contains potassium phosphate buffer salts and BSA as stabilizer.
Weitere Informationen über Enzyme für die Analyse komplexer Kohlenhydrate finden Sie unter www.sigma-aldrich.com/enzymeexplorer

Eignung

Depending on reaction conditions, digestion of chondroitin sulfate may result in hydrolysis of disaccharides

Einheitendefinition

One unit will cause a ΔA232 of 1.0 per minute due to the release of unsaturated disaccharide from chondroitin sulfate A at pH 7.3 at 37 °C. Reaction volume: 3.1 ml (light path 1 cm).

Angaben zur Herstellung

Reconstitute the product in 20 mM phosphate buffer, pH 7.0. Subsequent dilutions can be made with a 0.01% aqueous bovine serum albumin solution.

Anwendung

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Florian D Naal et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 87(1), 19-25 (2008-03-25)
Meniscal allograft processing procedures, in particular gamma irradiation, deteriorate the biomechanical and biological properties of the transplanted tissue. High hydrostatic pressure (HHP) treatment, widely used in food technology to inactivate microorganisms while preserving natural compounds, might serve as a gentle
Jessica S Kim et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(8), 780-790 (2011-08-02)
Cutaneous lupus erythematosus and dermatomyositis (DM) are chronic inflammatory diseases of the skin with accumulated dermal mucin. Earlier work has shown chondroitin sulfate (CS) accumulation within the dermis of discoid lupus erythematosus (DLE), subacute cutaneous lupus erythematosus (SCLE), and DM
E B Hunziker
Osteoarthritis and cartilage, 9(1), 22-32 (2001-02-17)
We have previously shown (Hunziker and Rosenberg, J Bone Joint Surg 1996;78A:721-33) that synovial cells can be induced to migrate into partial-thickness articular cartilage defects, therein to proliferate and subsequently to deposit a scar-like tissue. We now wished to ascertain
C B Knudson et al.
The Journal of cell biology, 100(5), 1753-1758 (1985-05-01)
Hyaluronate levels change dramatically during morphogenesis of various tissues and organs. Morphological detection of the exact temporal and spatial distribution patterns of hyaluronate may help to elucidate its role in morphogenesis. Since no specific direct method for visualizing hyaluronate with
S P Levine et al.
Blood, 75(4), 902-910 (1990-02-15)
Platelet factor 4 (PF4) is a hydrophobic, alpha-granule protein with potent antiheparin activity. It also binds to a chondroitin sulfate-containing proteoglycan (PG) isolated from platelets. In order to evaluate further the relationship between PF4 and the chondroitin sulfate-containing proteoglycan in
Alle zugehörigen Dokumente anzeigen

Artikel

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.