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Merck

C0543

Sigma-Aldrich

Rat Collagen Type IV

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

Synonym(e):

Type IV collagen, EHS-collagen

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About This Item

CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.75

product name

Kollagen aus Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

Biologische Quelle

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

Qualitätsniveau

Typ

Type IV (Miller)

Produktlinie

BioReagent

Form

lyophilized powder

Verpackung

glass bottle of 1 vial

Methode(n)

cell culture | mammalian: suitable

Oberflächendeckung

6‑10 μg/cm2

Löslichkeit

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Allgemeine Beschreibung

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Anwendung

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:
  • in adhesion assays.
  • in hydrogel fabrication.
  • to coat cell culture plates for cell differentiation.
  • to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.
Recommended for use as a cell culture substratum at 6-10 μg/cm2.

Biochem./physiol. Wirkung

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Sonstige Hinweise

Native collagen
We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Menge

0.75 mg/vial

Angaben zur Herstellung

Chloroform treated.
Pepsin is not used in the preparation.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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