B4062

BRAF (416-766), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): BRAF1, MGC126806, MGC138284, RAFB1
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in baculovirus infected Sf9 cells
• Qualitätsniveau: 200
• Produktlinie: PRECISIO^® Kinase
• Assay: ≥70% (SDS-PAGE)
• Form: buffered aqueous glycerol solution
• Spezifische Aktivität: 63-85 nmol/min·mg
• Mol-Gew.: ~63 kDa
• UniProt-Hinterlegungsnummer: P15056
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... BRAF(673)

Beschreibung

Biochem./physiol. Wirkung

BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRAF in cells responding to growth factor signals. Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 599 within the activation segment of BRAF.

Physikalische Form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Rechtliche Hinweise

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)