B0287

Monoclonal Anti-FITC−Biotin antibody produced in mouse

clone FL-D6, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Monoclonal Anti-FITC
• UNSPSC-Code: 12352203
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: biotin conjugate
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: FL-D6, monoclonal
• Form: buffered aqueous solution
• Methode(n): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using human tonsil
• Isotyp: IgG1
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-FITC (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Spezifität

The antibody reacts with both free and conjugated fluorescein isothiocyanate (FITC).

Immunogen

FITC-BSA conjugate

Anwendung

Monoclonal Anti-FITC-Biotin antibody produced in mouse has been used:

• for amplification of signal in immunofluorescence assays
• in Ag-activated beads for flow-based micro immunoassay of parathyroid hormone and IL-5
• enzyme linked immunosorbent assay (ELISA)

Biochem./physiol. Wirkung

Fluorochrome labeling provides a rapid and accurate localization of the site of antigen-antibody interaction when one of the reactants from parts of a cell, tissue or other biological structure. Fluorescein isothiocyanate (FITC) is a commonly used marker for antibodies in immunofluorescence techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labelled substances. FITC is widely used as a hapten to label different proteins and anti-FITC antibodies are used to identify these labeled proteins. Antibodies to FITC serve as universal indicator reagents by bridging FITC with another immunohistochemical reagent, such as alkaline phosphatase, horseradish peroxidase or biotin.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable