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Merck

A3352

Sigma-Aldrich

Acyl-Coenzym A-Synthetase aus Pseudomonas sp.

≥2 units/mg protein

Synonym(e):

Acid: CoA ligase (AMP-forming)

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About This Item

CAS-Nummer:
EC-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204

Form

powder

Qualitätsniveau

Spezifische Aktivität

≥2 units/mg protein

Versandbedingung

dry ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

Acyl-coenzyme A synthetase belongs to adenylate-forming enzymes superfamily. It has a conserved adenosine triphosphate/adenosine monophosphate (ATP/AMP) binding motif.

Anwendung

Acyl-coenzyme A (coA) synthetase from Pseudomonas sp. has been used:
  • as ligase in the synthesis of mevalonate derivatives of adenosine triphosphate (ATP)
  • in in vitro fatty acylation assays
  • in the synthesis of (14C)Vernoloyl-CoA (Va-CoA),(3) bisphosphonates derivatives of ATP(4) and (3H)Palmitate-CoA

Acyl-coenzyme A Synthetase may be used to study fatty acid metabolism and lipid metabolism. It has been used to study its interaction with fatty acid transport proteins, which has been found to be involved in the efficient cellular uptake of long-chain fatty acids in adipocytes .

Biochem./physiol. Wirkung

Acyl coenzyme A synthetase proteins are involved in regulating and facilitating long-chain fatty acid transport in mammalian cells .
Acyl-coenzyme A (CoA) synthetase (ACS) enzyme catalyzes a two-step thioesterification reaction involving the conversion of free fatty acids (FAs) to CoA esters. The substrate for ACS varies from two carbon to 26 carbon FAs. It is involved in the activation of FAs for lipid metabolism and enables FA to participate in various cellular metabolic pathways.

Einheitendefinition

1 U bildet 1.0 μmol AMP und Oleoyl-Coenzym A aus ATP und Oleat bei pH 8.1 und 25 °C in Anwesenheit von Coenzym A.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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