90102522

Hak cell line

• UNSPSC-Code: 41106514
• NACRES: NA.81

Eigenschaften

• Biologische Quelle: hamster kidney
• Qualitätsniveau: 100
• Form: liquid
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 56
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Hamster Syrian kidney

Beschreibung der Zelllinie

From 2 normal young adult males. Capable of growth in suspension (spinner) cultures. Useful for growth of several viruses including arboviruses, Coxsackie A4, A8, B1, HSV, smallpox and Asian strain influenza.

Anwendung

Virus studies: Arboviruses, Coxsackie A4, A8, B1, HSV, smallpox influenza virus

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% tr

Sonstige Hinweise

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Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable