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Merck

78102

Sigma-Aldrich

Anti-Maus-IgG – Atto 633 in Ziege hergestellte Antikörper

~1 mg/mL IgG, affinity isolated antibody

Synonym(e):

Atto 633 [Ziege-Anti-Maus IgG]

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

goat

Qualitätsniveau

Konjugat

Atto 633 conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Enthält

50% glycerol as stabilizer

Speziesreaktivität

mouse

Konzentration

~1 mg/mL IgG

Fluoreszenz

λex 636 nm; λem 650 nm in PBS

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Anti-mouse IgG (whole molecule) is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 633 NHS and purified by gel permeation chromatography and dialysis to remove unbound dye. Atto 633-goat anti mouse IgG associates with mouse IgGs.

Immunogen

purified mouse IgG (whole molecule).

Anwendung

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spect

Physikalische Form

Solution in phosphate buffered saline and 5 mM sodium azide, pH 7.5, with glycerol 1:1

Hinweis zur Analyse

Unconjugated dye ≤5% of total fluorescence

Rechtliche Hinweise

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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H-Sätze

P-Sätze

Gefahreneinstufungen

Aquatic Chronic 3

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Charlotte Calvet et al.
iScience, 25(12), 105628-105628 (2022-12-10)
Hearing depends on fast and sustained calcium-dependent synaptic vesicle fusion at the ribbon synapses of cochlear inner hair cells (IHCs). The implication of the canonical neuronal SNARE complex in this exocytotic process has so far remained controversial. We investigated the
Tharun Selvam Mahendran et al.
Frontiers in aging neuroscience, 12, 537712-537712 (2020-12-01)
Aggregated tau is a hallmark neuropathological feature in numerous neurodegenerative disorders. Previous studies aiming to validate aggregated tau pathology as a pathogenic driver of neurodegeneration in correlation to characteristic behavioral phenotypes have had shortcomings. Although studies on soluble tau pathology
Complement-fixing IgG1 constitutes a new subclass of mouse IgG.
P L Ey et al.
Nature, 281(5731), 492-493 (1979-10-11)
Structure and function of human and murine receptors for IgG.
J C Unkeless et al.
Annual review of immunology, 6, 251-281 (1988-01-01)
Jian Zhang et al.
International journal of oncology, 45(2), 683-690 (2014-06-04)
Tanshinone IIA (TSIIA), a natural diterpene quinone in the traditional Chinese medicinal herb Dan-Shen (Salvia miltiorrhiza), has extensively exerted antitumor activity in cellular and animal models. However, the molecular mechanisms underlying the antitumor effects of TSIIA remain largely unknown. The

Artikel

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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