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50283

Sigma-Aldrich

Anti-Maus-IgG - Atto 655 in Ziege hergestellte Antikörper

~1 mg/mL IgG

Synonym(e):

Atto 655-[Ziege-Anti-Maus IgG]

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

goat

Rekombinant

expressed in HEK 293 cells

Konjugat

Atto 655 conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Enthält

50% glycerol

Speziesreaktivität

mouse

Konzentration

~1 mg/mL IgG

Methode(n)

immunofluorescence: suitable

Fluoreszenz

λex 655 nm; λem 677 nm in PBS

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Anti-mouse IgG (whole molecule) (Sigma, M8645) is developed in goat using purified mouse IgG as the immunogen. Affinity isolated antigen specific antibody is purified from goat anti-mouse IgG antiserum to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity. Goat anti-mouse is conjugated to Atto 655 NHS (Abs.max. 663 nm; Em.max. 684 nm),(Cat. No. 76245), then further purified via gel permeation chromatography and dialysis to remove unbound Atto-Dye.

Immunogen

purified mouse IgG

Anwendung

Atto 655-goat anti-Mouse IgG - Atto 655 antibody has been used for immunofluorescence applications.

Verpackung

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Hinweis zur Analyse

frei von unkonjugiertem Farbstoff

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H319

Gefahreneinstufungen

Eye Irrit. 2

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

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Wilmes, S.; Staufenbiel, M.; Lisse, D.; Richter, Ch. P.; Beutel, O.; Busch, K. B. et al.
Angewandte Chemie (International Edition in English), 124(20), 4952-4955 (2012)
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Artikel

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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