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Sigma-Aldrich

5-Carboxy-X-rhodamin-N-succinimidylester

BioReagent, suitable for fluorescence

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About This Item

Empirische Formel (Hill-System):
C37H33N3O7
CAS-Nummer:
209734-74-7
Molekulargewicht:
631.67
MDL-Nummer:
MFCD07437880
UNSPSC-Code:
12352116
PubChem Substanz-ID:
329756168
NACRES:
NA.32

Produktlinie

BioReagent

Form

powder

Löslichkeit

DMF: soluble
acetonitrile: soluble

Fluoreszenz

λex 575 nm; λem 600 nm in 0.1 M phosphate pH 7.0

Eignung

suitable for fluorescence

Lagertemp.

−20°C

SMILES String

[O-]C(=O)c1cc(ccc1-c2c3cc4CCCN5CCCc(c45)c3[o+]c6c7CCCN8CCCc(cc26)c78)C(=O)ON9C(=O)CCC9=O

InChI

1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(26(19-22)36(43)44)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2

InChIKey

BTTBJYLMDASSAS-UHFFFAOYSA-N

Anwendung

5-Carboxy-X-rhodamine N-succinimidyl ester (ROX-SE) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications.Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing.

Sonstige Hinweise

Labeling of oligonucleotides

Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves

Analysenzertifikate (COA)

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Jonathan F Lovell et al.
Journal of the American Chemical Society, 133(46), 18580-18582 (2011-10-27)
Photodynamic therapy (PDT) stands to benefit from improved approaches to real-time treatment monitoring. One method is to use activatable photosensitizers that can both induce cell death (via singlet oxygen) and monitor it (via caspase detection). Here, we report porphyrins as
Dong-Shan Xiang et al.
The Analyst, 137(16), 3787-3793 (2012-07-06)
We have developed a dual color fluorescence quantitative detection method for specific single-stranded DNA with molecular beacons (MBs) and nucleic acid dye SYBR Green I by synchronous scanning fluorescence spectrometry. It is demonstrated by a reverse-transcription oligonucleotide sequence (target DNA
Yong Huang et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 17(26), 7313-7319 (2011-05-11)
A highly sensitive and selective assay based on a novel enzyme-responsive multicolor gold nanobeacon has been developed for the multiplex detection of endonucleases, a group of very important nucleases. The nanobeacon takes advantage of the high specificity of DNA cleavage
Xia Xiang et al.
Analytica chimica acta, 751, 155-160 (2012-10-23)
The development of simple and inexpensive DNA detection strategy is very significant for droplet-based microfluidic system. Here, a droplet-based biosensor for multiplexed DNA analysis is developed with a common imaging device by using fluorescence-based colorimetric method and a graphene nanoprobe.
Ting Qiu et al.
The Analyst, 135(9), 2394-2399 (2010-08-03)
A new strategy for quantitatively detecting micrococcal nuclease (MNase) is proposed using electrostatic interaction-based fluorescence resonance energy transfer (FRET) between positively charged QDs and negatively charged dye-labeled single-stranded DNA (dye-ssDNA). Herein, we have made our attempt to develop a strategy
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