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21955

Sigma-Aldrich

5(6)-Carboxytetramethylrhodamin-N-succinimidylester

BioReagent, suitable for fluorescence, ≥70% (coupling to amines)

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About This Item

Empirische Formel (Hill-System):
C29H25N3O7
CAS-Nummer:
150408-83-6
Molekulargewicht:
527.52
Beilstein:
8739319
MDL-Nummer:
MFCD00077324
UNSPSC-Code:
12352116
PubChem Substanz-ID:
57647956
NACRES:
NA.32

Produktlinie

BioReagent

Assay

≥70% (coupling to amines)

Form

powder

Löslichkeit

DMF: soluble
acetonitrile: soluble
methanol: soluble

Fluoreszenz

λex 543 nm; λem 576 nm in methanol
λex 554 nm; λem 584 nm in 0.1 M phosphate pH 8.0

Eignung

suitable for fluorescence

Lagertemp.

−20°C

SMILES String

CN(C)c1ccc2c(Oc3cc(ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)N(C)C)c1

InChI

1S/C29H25N3O7/c1-30(2)17-6-9-21-23(14-17)37-24-15-18(31(3)4)7-10-22(24)29(21)20-8-5-16(13-19(20)28(36)38-29)27(35)39-32-25(33)11-12-26(32)34/h5-10,13-15H,11-12H2,1-4H3

InChIKey

CXYYHBMOVJJZTD-UHFFFAOYSA-N

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Anwendung

5(6)-Carboxytetramethylrhodamine N-succinimidyl ester (TMR-SE) is used as an amine coupling reagent to form 5(6)-carboxytetramethylrhodamine (TMR) derivatized compounds such as proteins and drugs.

Verpackung

Bottomless glass bottle. Contents are inside inserted fused cone.

Sonstige Hinweise

Rhodamine derived label for amine modification and protein conjugation

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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L D Mayfield et al.
Bioorganic & medicinal chemistry letters, 9(10), 1419-1422 (1999-06-09)
Resins for solid-phase synthesis can affect coupling efficiencies by interacting with reactants. We have observed that polyethylene glycol-polystyrene (PEG-PS) solid support absorbs added activated fluorophores, preventing efficient labeling of peptide nucleic acids (PNAs). We now report that addition of an
Divyamani Srinivasan et al.
PloS one, 6(3), e17732-e17732 (2011-03-23)
Cell-penetrating peptides (CPPs) can transport macromolecular cargos into live cells. However, the cellular delivery efficiency of these reagents is often suboptimal because CPP-cargo conjugates typically remain trapped inside endosomes. Interestingly, irradiation of fluorescently labeled CPPs with light increases the release
E. Koller
Appl. Fluoresc. Technol., 3, 20-20 (1991)
P Kask et al.
Biophysical journal, 78(4), 1703-1713 (2000-03-29)
A method of sample analysis is presented which is based on fitting a joint distribution of photon count numbers. In experiments, fluorescence from a microscopic volume containing a fluctuating number of molecules is monitored by two detectors, using a confocal
Yoshiaki Yano et al.
Biochemistry, 41(9), 3073-3080 (2002-02-28)
Investigation of interactions between hydrophobic model peptides and lipid bilayers is perhaps the only way to elucidate the principles of the folding and stability of membrane proteins (White, S. H., and Wimley, W. C. (1998) Biochim. Biophys. Acta 1367, 339-352).
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