DCTP-RO

2′-Desoxycytidin-5′-triphosphat Dinatriumsalz

99% (dNTP), <0.9% (dNDP), solution, 100 mM

• Synonym(e): dCTP-Na₂
• Empirische Formel (Hill-System): C₉H₁₄N₃Na₂O₁₃P₃
• CAS-Nummer: 102783-51-7
• Molekulargewicht: 511.12
• MDL-Nummer: MFCD00084683
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 329798916

Eigenschaften

• Beschreibung: PCR Grade, sodium salt
• Qualitätsniveau: 100
• Assay: 99% (dNTP)
• Form: solution
• Verwendung: sufficient for 125,000 standard reactions (03732690001); sufficient for 31,250 standard reactions (11969021001); sufficient for 6,250 standard reactions (11934520001)
• Verpackung: pkg of 1,250 μL (11969021001 [100 mM]); pkg of 4 × 1,250 μL (03732690001 [4x100 mM]); pkg of 250 μL (11934520001 [100 mM])
• Hersteller/Markenname: Roche
• Konzentration: <0.9% (dNDP); 100 mM
• Lagertemp.: −20°C
• SMILES String: [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
• InChI: 1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
• InChIKey: ABWVCNMFYVEBIB-CDNBRZBRSA-L

Beschreibung

Allgemeine Beschreibung

dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Anwendung

2′-Desoxycytidin-5′-triphosphat-dinatriumsalz ist eines der Nukleotidtriphosphate, das für durch DNA-Polymerase ausgelöste Reaktionen wie z. B. Polymerase-Kettenreaktion, DNA-Sequenzierung und andere molekularbiologische Methoden verwendet wird.

Qualität

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physikalische Form

solution of dCTP in water (pH 8.3).

Angaben zur Herstellung

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Sonstige Hinweise

For general laboratory use.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash