11051466001

2′-Desoxyguanosin 5′-triphosphat Dinatriumsalz

=98%, solution, 100 mM, suitable for PCR, suitable for DNA sequencing

• Synonym(e): dGTP-Na₃
• Empirische Formel (Hill-System): C₁₀H₁₃N₅Na₃O₁₃P₃
• CAS-Nummer: 93919-41-6
• Molekulargewicht: 573.13
• MDL-Nummer: MFCD00080337
• UNSPSC-Code: 41116100
• PubChem Substanz-ID: 329749301

Eigenschaften

• Qualitätsniveau: 100
• Beschreibung: Lithium salt
• Assay: 98%
• Form: solution
• Verwendung: sufficient for 6,250 standard reactions (PCR)
• Verpackung: pkg of 250 μL (25 μmol; 100 mM)
• Hersteller/Markenname: Roche
• Konzentration: 100 mM
• Methode(n): DNA amplification: suitable; DNA sequencing: suitable; DNA synthesis: suitable; PCR: suitable
• Lagertemp.: −20°C
• SMILES String: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
• InChI: 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
• InChIKey: IWGGLKOTEOCWQP-BIHLCPNHSA-K

Beschreibung

Allgemeine Beschreibung

2′-Desoxyguanosin-5′-triphosphat (dGTP) besteht aus einer Guaninnukleobase, die an Desoxyribose gebunden ist, und einem 5‘-Hydroxyl an dem Zucker, der an eine Kette aus drei Phosphatsresten gebunden ist. Es wird von Zellen für die Synthese von DNA mithilfe von DNA-Polymerase verwendet.

dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dGTP solution in water (pH 7), 100 mM, lithium salt.

Anwendung

2′-Desoxyguanosin 5′-triphosphat-Trinatriumsalz wird bei Anwendungen wie der DNA-Sequenzierung, PCR und anderen DNA-Polymerase-basierten DNA-Synthesen und Reparaturmethoden eingesetzt.

Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

Qualität

Typical analysis:

• ≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC)
• Absorbance determination A₂₅₀/A₂₆₀, A₂₈₀/A₂₆₀, A₂₉₀/A₂₆₀
• DNase activity negative, according to the current Quality Control procedures
• Suitability for PCR

Einheitendefinition

1 μmol dGTP = 13.7 A₂₅₂ units

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:

• nucleotide sets (all 4 nucleotides in separate vials)
• ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:

• kits (dNTPs, buffer, and polymerase in separate vials)
• ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): No data available
• Flammpunkt (°C): No data available