11426338910

Anti-Fluorescein-AP, Fab fragments

from sheep

• Synonym(e): antibody
• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: sheep
• Qualitätsniveau: 100
• Konjugat: alkaline phosphatase conjugate
• Antikörperform: affinity purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: solution
• Verpackung: pkg of 150 U (200 μl)
• Hersteller/Markenname: Roche
• Isotyp: IgG
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)

Spezifität

The polyclonal antibody reacts with free and bound fluorescein.

Anwendung

Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:

• Dot blot
• ELISA
• Immunohistocytochemistry
• In situ hybridization
• Southern blot
• Western blot
• Electrochemical biosensing technique for the detection of 16S rRNA

Biochem./physiol. Wirkung

Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).

Leistungsmerkmale und Vorteile

Contents
Solution, stabilized

Angaben zur Herstellung

• Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
• ELISA: 150 to 300mU/ml
• Immunohistocytochemistry: 250 to 500mU/ml
• In situ hybridization: 1.5 to 7.5U/ml
• Southern blot: 150mU/ml
• Western blot: 250 to 500 mU/ml

Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H317
• P-Sätze: P261 - P272 - P280 - P333 + P313 - P362 + P364 - P501
• Gefahreneinstufungen: Skin Sens. 1
• WGK: WGK 1
• Flammpunkt (°F): No data available
• Flammpunkt (°C): No data available