10634425001

n-Octylglucosid

non-ionic

• Synonym(e): Octyl-β-D-Glucopyranosid, n-Octyl-glucosid, OGP
• Empirische Formel (Hill-System): C₁₄H₂₈O₆
• CAS-Nummer: 29836-26-8
• Molekulargewicht: 292.37
• Beilstein: 84118
• MDL-Nummer: MFCD00063288
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 329748920

Eigenschaften

• Assay: 99% (GC)
• Mol-Gew.: micellar avg mol wt 25,000; 292.4
• Verpackung: pkg of 10 g
• Hersteller/Markenname: Roche
• Aggregatnummer: 84
• Methode(n): dialysis: suitable
• CMC: 20-25 mM (20-25°C)
• Übergangstemp.: cloud point >100 °C
• Versandbedingung: ambient
• SMILES String: CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
• InChI: 1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1
• InChIKey: HEGSGKPQLMEBJL-RKQHYHRCSA-N

Beschreibung

Allgemeine Beschreibung

1-O-n-Octyl-β-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.

Anwendung

Nicht-ionisches, dialysierbares Waschmittel für die Auflösung und Isolation von Membranproteine. Es zeigte sich eine Erhöhung der Resolution der Proteine im 2D-Gele.

N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It is a mild and non-denaturing detergent for the solubilization and reconstitution of membrane proteins. N-Octylglucoside is easily removed by dialysis. N-Octylglucoside has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.

Qualität

Purity: 99% (from C), homogeneous (GC)

Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C₁₄H₂₈O₆

Angaben zur Herstellung

Working concentration: 46 mM
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.

Lagerung und Haltbarkeit

Store at 15 to 25 °C. (Store dry!)

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable