10378445001

Chromozym PK

90% (enzymatic), powder, pkg of 20 mg

• Synonym(e): C33H39N8O6COOCH3
• UNSPSC-Code: 12352204

Eigenschaften

• Beschreibung: Molecular Formula: C₃₃H₃₉N₈O₆COOCH₃
• Qualitätsniveau: 100
• Assay: 90% (enzymatic)
• Form: powder
• Mol-Gew.: M_r 702.9
• Verpackung: pkg of 20 mg
• Hersteller/Markenname: Roche
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Chromozym^® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.

Anwendung

Use Chromozym^® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.

Qualität

Contaminant: <0.5% free 4-nitraniline

Sequenz

Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate

Prinzip

Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.

Angaben zur Herstellung

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)
Working concentration: Approximately 0.5 mM
Storage conditions (working solution): 2 to 8 °C
An aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Rechtliche Hinweise

Chromozym is a registered trademark of Pentapharm AG, Basel

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable