SCR078
Fluorescent Human ES/iPS Cell Characterization Kit
This Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human Embryonic stem & induced pluripotent Stem cells.
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About This Item
UNSPSC-Code:
12161503
eCl@ss:
32161000
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Allgemeine Beschreibung
Human embryonic stem (hES) cells are pluripotent cells derived from the inner cell mass of pre-implantation blastocysts. Human induced pluripotent (hiPS) cells are pluripotent cells generated by reprogramming human somatic cells using four transcription factors, Oct-4, Klf-4, Sox-2, and c-Myc, or their variants. Both hESC and hiPSC can self-renew and have the ability to generate all three germ layers: ectoderm, mesoderm, and endoderm. In vitro, hESC/iPSC are normally maintained and propagated on mouse fibroblast feeders for extended periods in media containing basic fibroblast growth factor (bFGF). However, spontaneous differentiation may occur in subpopulations of cells. Several pluripotent markers are commonly used to distinguish pluripotent hESC/iPSC from differentiated cells.
EMD Millipore’s Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human ES/iPS cells. Included in the kit is an enzymatic assay to measure alkaline phosphatase activity in the cells along with validated directly conjugated antibodies to pluripotent transcription factors, Oct-4, Sox-2 and Nanog and cell surface epitopes TRA-1-60 and TRA-1-81 to enable rapid immunocytochemical marker analysis. The Dapi nuclear dye is conveniently included to aid in cell quantification. While the expression levels of pluripotent markers are expected to be diminished upon differentiation, each possess specific expression kinetics. For example, it has been noted that upon differentiation, Oct-4 and TRA-1-60 expressions are the first to be down-regulated while Nanog and alkaline phosphatase down-regulate at a much slower timeframe.
EMD Millipore’s Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human ES/iPS cells. Included in the kit is an enzymatic assay to measure alkaline phosphatase activity in the cells along with validated directly conjugated antibodies to pluripotent transcription factors, Oct-4, Sox-2 and Nanog and cell surface epitopes TRA-1-60 and TRA-1-81 to enable rapid immunocytochemical marker analysis. The Dapi nuclear dye is conveniently included to aid in cell quantification. While the expression levels of pluripotent markers are expected to be diminished upon differentiation, each possess specific expression kinetics. For example, it has been noted that upon differentiation, Oct-4 and TRA-1-60 expressions are the first to be down-regulated while Nanog and alkaline phosphatase down-regulate at a much slower timeframe.
Spezifität
Cross Reactivty
None
None
Anwendung
Research Category
Stammzellforschung
Stammzellforschung
Research Sub Category
Pluripotente Stammzellen & Stammzellen der frühen Entwicklungsphase
Pluripotente Stammzellen & Stammzellen der frühen Entwicklungsphase
This Fluorescent Human ES/iPS Cell Characterization Kit contains a range of sensitive tools for the phenotypic assessment of the pluripotent status of human Embryonic stem & induced pluripotent Stem cells.
Komponenten
1. Fast Red Violet solution (Part No. 90239): One 15 mL bottle.
2. Napthol AS-BI phosphate solution (Part No. 90234). One 15 mL bottle.
3. Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor™ 488 conjugate (Part No. MAB4419A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
4. Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate (Part No. MAB4423C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
5. Mouse anti-Nanog, clone 7F7.1, Alexa Fluor 488 conjugate (Part No. MABD24A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
6. Mouse anti-TRA-1-60, clone TRA-1-60, Cy3 conjugate (Part No. MAB4360C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
7. Mouse anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate (Part No. MAB4381C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
8. DAPI, 100 µL (Part No. 90229). One vial containing 100 µL volume.
2. Napthol AS-BI phosphate solution (Part No. 90234). One 15 mL bottle.
3. Mouse anti-Oct-4 (POU5f1), clone 7F9.2, Alexa Fluor™ 488 conjugate (Part No. MAB4419A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
4. Mouse anti-Sox-2, clone 10H9.1, Cy3 conjugate (Part No. MAB4423C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
5. Mouse anti-Nanog, clone 7F7.1, Alexa Fluor 488 conjugate (Part No. MABD24A4-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
6. Mouse anti-TRA-1-60, clone TRA-1-60, Cy3 conjugate (Part No. MAB4360C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
7. Mouse anti-TRA-1-81, clone TRA-1-81, Cy3 conjugate (Part No. MAB4381C3-50UL). One vial containing 50 µL of 0.5 mg/mL conjugated monoclonal antibody.
8. DAPI, 100 µL (Part No. 90229). One vial containing 100 µL volume.
Qualität
Conjugated antibodies and alkaline phosphatase reagents have been validated on H9 human ES and STEMCCA derived human ips cells.
Physikalische Form
Purified conjugated mouse monoclonal antibodies are stored in PBS with 0.1% sodium azide and 15mg/ml BSA.
Lagerung und Haltbarkeit
Store at 2-8°C until expiration date of kit. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
Rechtliche Hinweise
ALEXA FLUOR is a trademark of Life Technologies
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Carc. 2 - Eye Irrit. 2 - Skin Irrit. 2
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Analysenzertifikate (COA)
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Melissa Conti Mazza et al.
Stem cell research, 55, 102506-102506 (2021-08-23)
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