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PC176

Sigma-Aldrich

Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb

liquid, Calbiochem®

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About This Item

UNSPSC-Code:
12352203

Biologische Quelle

rabbit

Qualitätsniveau

100

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

liquid

Enthält

≤0.1% sodium azide as preservative

Speziesreaktivität

mouse, sheep, rat

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze
avoid repeated freeze/thaw cycles

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

rat ... Htr2A(29595)

Allgemeine Beschreibung

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
This Anti-Serotonin 2A Receptor Rabbit pAb is validated for use in Frozen Sections, WB, Free Floating Sections, Paraffin Sections for the detection of Serotonin 2A Receptor.

Immunogen

Rat
a synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor

Anwendung


Frozen Sections (1:300-1:500)
Immunoblotting (1:500-1:1000)
Free Floating Sections (see application references)
Paraffin Sections (see application references; heat pre-treatment required)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

In 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Sonstige Hinweise

Antibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
Leonard, B.E. 1996. Psychother. Psychosom.65, 66.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol.8, 175.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Gongliang Zhang et al.
Neuropharmacology, 109, 332-340 (2016-04-27)
The rodent hippocampus supports non-spatial object memory. Serotonin 5-HT2A receptors (5-HT2AR) are widely expressed throughout the hippocampus. We previously demonstrated that the activation of 5-HT2ARs enhanced the strength of object memory assessed 24 h after a limited (i.e., weak memory) training
Youngjae Ryu et al.
Scientific reports, 11(1), 7120-7120 (2021-03-31)
In the majority of spinal cord injury (SCI) patients, spasticity develops in the subacute phase and chronically persists with muscle hypertonia. Among various pathological conditions underlying spasticity, upregulated expression of 5-HT receptors (5-HTR) on the spinal motor neurons due to
Lizhu Jiang et al.
Frontiers in molecular neuroscience, 12, 317-317 (2020-02-11)
Spaced training is robustly superior to massed training, which is a well-documented phenomenon in humans and animals. However, the mechanisms underlying the spacing effect still remain unclear. We have reported previously that spacing training exerts memory-enhancing effects by inhibiting forgetting

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