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Merck

MAC112

Sigma-Aldrich

Anti-S•Tag fusion protein Antibody

clone 26A4.1.2, from mouse

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

26A4.1.2, monoclonal

Speziesreaktivität (Voraussage durch Homologie)

all

Methode(n)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1κ

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

The S•Tag fusion protein is one of many polypeptide tags used to facilitate the identification and purification of many targets of interest. The gene encoding the S•Tag peptide can be added into a vector with another gene that encodes a target of interest. Such vectors, which are available through the Novagen product line, are subsequently transfected into various mammalian, bacterial, or insect cell cultures. Because the target of interest and the S•Tag peptide are expressed as one protein, identification of the target is facilitated with the use of antibodies that are specific for the respective fusion tag. Optional purification of the target may be performed using chromatography techniques that allow for the binding and removal of the respective fusion tag, if desired.

Immunogen

Linear peptide corresponding to S•Tag fusion proteins.

Anwendung

Research Category
Epitop-Tags & allgemeine Anwendung
Research Sub Category
Epitop-Tags
Detect S•Tag fusion protein using this Anti-S•Tag fusion protein Antibody validated for use in Western Blotting, ICC, Immunoprecipitation.
Immunocytochemistry Analysis: A representative lot detected S•Tag fusion proteins in transfected COS-7 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitate S•Tag fusion proteins in IP.

Qualität

Evaluated by Western Blot in Posi-Tag Epitope Tag Control.

Western Blot Analysis: 0.05 µg/mL of this antibody detected S•Tag fusion proteins in 5 µL of Posi-Tag Epitope Tag Control.

Zielbeschreibung

~45 kDa observed. The molecular weight of S•Tag fusion protein is not 45 kDa The Posi-Tag Epitope Tag Control, which contains an S•Tag fusion protein, is observed at ~45 kDa

Physikalische Form

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
Posi-Tag Epitope Tag Control

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Bandarigoda Nipunika Somatilaka et al.
Developmental cell, 54(6), 710-726 (2020-07-24)
The mechanisms underlying subcellular targeting of cAMP-generating adenylyl cyclases and processes regulated by their compartmentalization are poorly understood. Here, we identify Ankmy2 as a repressor of the Hedgehog pathway via adenylyl cyclase targeting. Ankmy2 binds to multiple adenylyl cyclases, determining

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