MABN1559

Anti-ATBF1 Antibody, clone 5A1.1

clone 5A1.1, from mouse

• Synonym(e): Zinc finger homeobox protein 3, AT motif-binding factor 1, AT-binding transcription factor 1, Alpha-fetoprotein enhancer-binding protein, Zinc finger homeodomain protein 3, ZFH-3
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 5A1.1, monoclonal
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25 μg
• Methode(n): western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_008816
• UniProt-Hinterlegungsnummer: Q15911
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ZFHX3(463)

Beschreibung

Allgemeine Beschreibung

Zinc finger homeobox protein 3 (UniProt: Q15911; also known as AT motif-binding factor 1, AT-binding transcription factor 1, ATBF1, Alpha-fetoprotein enhancer-binding protein, Zinc finger homeodomain protein 3, ZFH-3) is encoded by the ZFHX3 (also known as ATBF1) gene (Gene ID: 463) in human. ATBF1 contains 23 C2H2-type zinc fingers and 4 homeobox DNA-binding domains. It is shown to translocate from the cytoplasm to the nucleus following TGF-beta stimulation. It acts as a transcriptional regulator that can act as an activator or a repressor. It inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. In concert with SMAD-dependent TGF-beta signaling, it can repress the transcription of AFP via its interaction with SMAD2/3. ATBF1 is shown to regulate myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression. It is not found in normal gastric mucosa, but is found in gastric carcinoma cells and its expression is higher in ER-positive breast tumors than ER-negative breast tumors. It is shown to down-regulate the MUC5AC promoter in gastric cancer cells.

Spezifität

Clone 5A1.1 recognizes Zinc finger homeobox protein 3 (ATBF1) in human cells. It targets an epitope with in 87 amino acids from the C-terminal region. This antibody recognizes isoform of 306 and 404 kDa.

Immunogen

GST/His-tagged recombinant fragment corresponding to 87 amino acids from the C-terminal region of human Zinc finger homeobox protein 3.

Anwendung

Anti-ATBF1, clone 5A1.1, Cat. No. MABN1559, is a highly specific mouse monoclonal antibody that targets Zinc finger homeobox protein 3 and has been tested in Western Blotting.

Qualität

Evaluated by Western Blotting in SHSY-5Y cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected ATBF1 in 10 µg of SHSY-5Y cell lysate.

Zielbeschreibung

~404 kDa observed; 404.42 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Format: Purified

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1