Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

MABE50

Sigma-Aldrich

Anti-Phosphoepitope SR proteins Antibody, clone 1H4

clone 1H4, from mouse

Synonym(e):

Ser-Arg-rich proteins

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

100

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

1H4, monoclonal

Speziesreaktivität

rat

Speziesreaktivität (Voraussage durch Homologie)

human (based on 100% sequence homology), mouse (based on 100% sequence homology)

Methode(n)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1κ

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Ser-Arg-rich (SR) proteins make up a family of functionally and structurally conserved phosphoproteins, and are required components of alternative and constitutive pre-mRNA splicing. SR proteins are characterized by their modular composition consisting of two domains of interest: an N-terminal RNA recognition motif (RRM) which serves in the determination of DNA-binding specificity, and the RS domain, an arginine and serine rich, C-terminal domain that serves as a shuttling and localization director of SR proteins and as a splicing activation domain. They are involved in various aspects of pre-mRNA splicing and in spliceosome assembly including; the identification and appropriation of splice-sites, and the manipulation of alternative splicing regulation.

Immunogen

Purified oocyte nuclei containing xenopus SR proteins.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen
Research Sub Category
RNA-Stoffwechsel & Bindungsproteine
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC (Fukuhara, T., et al. (2006).

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP (Buratti, E., et al. (2007).
Use Anti-Phosphoepitope SR proteins Antibody, clone 1H4 (Mouse Monoclonal Antibody) validated in WB, ICC, IP to detect Phosphoepitope SR proteins also known as Ser-Arg-rich proteins.

Qualität

Evaluated by Western Blot in L6 plus insulin cell lysate.

Western Blot Analysis: 0.025 µg/mL of this antibody detected SR proteins in 10 µg of L6 plus insulin cell lysate.

Zielbeschreibung

~ 75, 55, 40, 30, and 20 kDa observed.
This antibody recognizes the family of SR proteins which consist of 75, 55, 40, 30, 20 kDa proteins.

Physikalische Form

Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
L6 plus insulin cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Produkt-Auswahlhilfe.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Yihui Shi et al.
PloS one, 15(5), e0233672-e0233672 (2020-05-30)
Agents that modulate pre-mRNA splicing are of interest in multiple therapeutic areas, including cancer. We report our recent screening results with the application of a cell-based Triple Exon Skipping Luciferase Reporter (TESLR) using a library that is composed of FDA
Anil Aktas Samur et al.
Blood cancer journal, 12(12), 171-171 (2022-12-20)
Splicing changes are common in cancer and are associated with dysregulated splicing factors. Here, we analyzed RNA-seq data from 323 newly diagnosed multiple myeloma (MM) patients and described the alternative splicing (AS) landscape. We observed a large number of splicing
Mousumi Khatun et al.
Hepatology (Baltimore, Md.), 74(1), 41-54 (2020-11-26)
HCV often causes chronic infection in liver, cirrhosis, and, in some instances, HCC. HCV encodes several factors' those impair host genes for establishment of chronic infection. The long noncoding RNAs (lncRNAs) display diverse effects on biological regulations. However, their role
Miriam Pacetti et al.
Disease models & mechanisms, 15(4) (2022-03-05)
The cellular level of TDP-43 (also known as TARDBP) is tightly regulated; increases or decreases in TDP-43 have deleterious effects in cells. The predominant mechanism responsible for the regulation of the level of TDP-43 is an autoregulatory negative feedback loop.
Amita Vaidya et al.
Cells, 9(8) (2020-08-07)
Breast tumor heterogeneity is a major impediment to oncotherapy. Cancer cells undergo rapid clonal evolution, thereby acquiring significant growth and invasive advantages. The absence of specific markers of these high-risk populations precludes efficient therapeutic and diagnostic management of the disease.
Alle zugehörigen Dokumente anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.