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Merck

MAB1393Z

Sigma-Aldrich

Anti-PECAM-1 Antibody, clone TLD-3A12, azide free

clone TLD-3A12, Chemicon®, from mouse

Synonym(e):

CD31, MAB1393

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

TLD-3A12, monoclonal

Speziesreaktivität

rat

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

Isotyp

IgG1

Eignung

not suitable for immunohistochemistry (Paraffin)

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Spezifität

Rat PECAM-1. Antibody gives positive reaction at high dilutions against recombinant rat PECAM-1, confirming specificity.

Immunogen

Activated, Lewis rat derived microglial cells.

Anwendung

Research Category
Zellstruktur
Research Sub Category
Adhäsions-Proteine (CAMs – Zelladhäsionsmoleküle)
Anti-PECAM-1 Antibody, clone TLD-3A12, azide free detects level of PECAM-1 & has been published & validated for use in ELISA, FC, WB, IH.
Flow Cytometry: 1:50-1:100

Immunohistology: frozen sections (-20°C methanol fixation), not recommended for traditional formalin fixed tissues.

ELISA

Western Blotting: 1:500. MW = 130-140 kDa

Optimal working dilutions must be determined by end user.

Physikalische Form

Format: Purified
Purified immunoglobulin by affinity chromatography on Protein G. Liquid in phosphate buffered saline containing no preservatives.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Hinweis zur Analyse

Control
POSITIVE CONTROL: Activated microglial cells.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Characterization of microglia and macrophages in the central nervous system of rats: definition of the differential expression of molecules using standard and novel monoclonal antibodies in normal CNS and in four models of parenchymal reaction.
Flaris, N A, et al.
Glia, 7, 34-40 (1993)
An interferon-inducible molecule on brain endothelium which controls lymphocyte adhesion mediated by integrins.
Male, D, et al.
Immunology, 84, 453-460 (1995)
D L Livant et al.
Cancer research, 60(2), 309-320 (2000-02-10)
Using naturally serum-free SU-ECM basement membranes as invasion substrates showed that plasma fibronectin was necessary to stimulate invasion by DU 145 human and metastatic MATLyLu (MLL) rat prostate carcinoma cells. This activity mapped to the PHSRN sequence, which induced invasion
Yingchao Shi et al.
PLoS biology, 18(5), e3000705-e3000705 (2020-05-14)
Modeling the processes of neuronal progenitor proliferation and differentiation to produce mature cortical neuron subtypes is essential for the study of human brain development and the search for potential cell therapies. We demonstrated a novel paradigm for the generation of

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