ABT22

Anti-Endophilin-A1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): SH3-domain GRB2-like 2, bA335L15.1 (SH3-domain GRB2-like 2), Endophilin A1 BAR domain, SH3 domain protein 2A, Endophilin-1, SH3 domain-containing GRB2-like protein 2
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: mouse, rat
• Methode(n): western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NP_062408
• UniProt-Hinterlegungsnummer: Q62420
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Sh3Gl2(20404)

Beschreibung

Allgemeine Beschreibung

A member of the Endophilin family, Endophilin A1 is a protein containing SH3 domains, and has been implicated in the trafficking of clathrin-mediated synaptic vesicle endocytosis within the membrane. It was originally characterized as a presynaptically enriched protein that binds to endocytic proteins polyphosphoinositide synaptojanin and dynamin. Endophilin A1 has been implicated in endocytic vesicle budding when its function in cell-free systems and in living synapses has been disturbed. Along with the SH3 domain, Endophilin A1 contains a BAR domain in the N-terminal region, that has been found to induce and bind membrane curvature. This protein’s expression is localized to the brain, most specifically in the frontal cortex. Markedly high expression is observed in fetal cerebellum.

Spezifität

Human (94% sequence homology).

The antibody recognizes Endophilin-A1.

Immunogen

KLH-conjugated linear peptide corresponding to mouse Endophilin-A1.

Anwendung

This Anti-Endophilin-A1 Antibody is validated for use in WB for the detection of Endophilin-A1.

Western Blot Analysis: 1:500 dilution from a previous lot detected Endophilin-A1 in 10 µg of rat brain and mouse brain membrane tissue lysates.

Qualität

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected Endophilin-A1 in 10 µg of mouse brain tissue lysate.

Zielbeschreibung

~ 42 kDa

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable