ABC517

Anti-PGAM5 Antibody

from rabbit, purified by affinity chromatography

• Synonym(e): Serine/threonine-protein phosphatase PGAM5, mitochondrial, Bcl-XL-binding protein v68, Phosphoglycerate mutase family member 5
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Aufgereinigt durch: affinity chromatography
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): primate (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rodent (based on 100% sequence homology), monkey (based on 100% sequence homology), hamster (based on 100% sequence homology)
• Methode(n): western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001164014
• UniProt-Hinterlegungsnummer: Q96HS1
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PGAM5(192111)

Beschreibung

Allgemeine Beschreibung

PGAM5 is a mitochondrial specific phosphatase specific for serine/threonine residues. PGAM5 principally dephosphorylates and activates MAP3K5 kinase a critical player in many cancers. PGAM5 also acts as a central mediator for programmed necrosis or necroptosis, induced by TNF, ROS or calcium. In the mitochondria, PGAM5 may also function as a regulator of mitochondrial dynamics and reconstruction. Also PGAM5 functions in the cellular stress response and regulates the removal of damaged mitochondria via mitophagy. PGAM5 itself is regulated at the protein level by the mitochondrial specific rhomboid protease PARL, which cleaves the N-terminal of PGAM5 in response to mitochondrial depolarization.

Spezifität

This antibody detects the -L(long) isoform and -S(short) isoform of PGAM5.

Immunogen

Epitope: Near N-terminal

KLH-conjugated linear peptide corresponding to human PGAM5 near the N-terminal.

Anwendung

Research Category
Apoptose & Krebs

Research Sub Category
Apoptose - Weiterführende Produkte

This Anti-PGAM5 Antibody is validated for use in Western Blotting for the detection of PGAM5.

Qualität

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PGAM5 in 10 µg of HT-29 cell lysate.

Zielbeschreibung

~32/34 kDa observed.

Physikalische Form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable