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Merck

AB5674P

Sigma-Aldrich

Anti-Acid Sensitive Ion Channel 1 Antibody

Chemicon®, from rabbit

Synonym(e):

ASIC1, BNC1, MDEG, BNaC1a, Amiloride-sensitive Cation Channel 2

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

rabbit

Qualitätsniveau

Antikörperform

affinity purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Aufgereinigt durch

affinity chromatography

Speziesreaktivität

rat, human

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable
western blot: suitable

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Spezifität

Recognizes rat Acid Sensitive Ion Channel 1 (ASIC1). The immunogen shows no significant sequence homology with ASIC-beta or other ASICs.

Immunogen

A 20 amino acid peptide sequence near the extracellular N-terminus of rat ASIC1 (Chen et al. 1998; Waldmann et al. 1997; Garcia-Anoveros et al. 1997; Waldmann et al. 1996).

The immunogen sequence has 100% homology with human sodium channel 2 or BNaC2.

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Ionenkanäle & -transporter
Detect Acid Sensitive Ion Channel 1 using this Anti-Acid Sensitive Ion Channel 1 Antibody validated for use in ELISA & WB.
Western blot: 1-10 μg/mL using ECL.

Immunohistochemistry: not tested. It is recommended that the antibody be tried at 1-20 μg/mL on paraformaldehyde fixed tissue.

ELISA: 0.5-1 μg/mL using 1 μg/mL control peptide (Catalog number AG394) per well.

Optimal working dilutions must be determined by the end user.

Physikalische Form

Affinity purified immunoglobulin. Liquid in phosphate buffered saline containing 0.1% BSA.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Neil D Detweiler et al.
Journal of applied physiology (Bethesda, Md. : 1985), 127(2), 393-407 (2019-06-07)
Acid-sensing ion channels (ASICs) are voltage-insensitive cation channels that contribute to cellular excitability. We previously reported that ASIC1 in pulmonary artery smooth muscle cells (PASMC) contribute to pulmonary vasoreactivity and vascular remodeling during the development of chronic hypoxia (CH)-induced pulmonary

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