684274

β1,2-Xylosidase, Xanthomonas sp.

• Synonym(e): β1,2-Xylosidase, Xanthomonas sp., β-D-Xylanxylohydrolase
• CAS-Nummer: 9025-53-0
• EC-Nummer: 3.2.1.37 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00130640
• UNSPSC-Code: 12352202
• NACRES: NA.54

Eigenschaften

• Form: liquid
• Qualitätsniveau: 100
• Spezifische Aktivität: ≥10 units/mL; ≥20 units/mg protein
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: do not freeze
• Fremdaktivität: β-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases, none detected
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Native β1,2-Xylosidase from Xanthomonas species. Catalyses the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of a1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Native β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.

Note: 1 mU = 1 milliunit.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.

Physikalische Form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Rekonstituierung

Following initial use, refrigerate (4°C).

Sonstige Hinweise

Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol.50, 1401.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable