614852

Topoisomerase IIα Inhibitor, TSC24

The Topoisomerase IIα Inhibitor, TSC24 controls the biological activity of Topoisomerase. This small molecule/inhibitor is primarily used for Cell Structure applications.

• Synonym(e): Topoisomerase IIα Inhibitor, TSC24, (E)-N,N-dimethyl-2-(quinolin-2-ylmethylene)hydrazinecarbothioamide
• Empirische Formel (Hill-System): C₁₃H₁₄N₄S
• Molekulargewicht: 258.34
• UNSPSC-Code: 12352200
• NACRES: NA.54

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥95% (HPLC)
• Form: powder
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Farbe: yellow
• Löslichkeit: DMSO: 15 mg/mL
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

A cell-permeable, TSC (thiosemicarbazone) derivative that acts as a selective and ATP competitive Topoisomerase IIα inhibitor and Fe chelator. Independent of its Fe chelating ability, this compound is shown to block Topo II&alpha-catalyzed ATP hydrolysis by competing with ATP for binding to the ATPase domain of Topo IIα (K_d = 18 µM). It also displays M phase cell-cycle arrest at 1 µM in nocodazole-treated HT-29 cells. This compound demonstrates anti-proliferative activity, without distinct or obvious selectivity, towards HeLa, HL-60, SGC7901, and HT-29 cultures (IC₅₀ = 90 nM, 50 nM, 30 nM, and 20 nM, respectively), and a broader panel of tumor cell lines with IC₅₀ values in the nanomolar range.

Warnhinweis

Toxicity: Standard Handling (A)

Rekonstituierung

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sonstige Hinweise

Huang, H., et al. 2010. J. Med. Chem.53, 3048.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable