1.05616

HPTLC plates, Silica gel F₂₅₄

pkg of 25 plates, plate L × W 5 cm × 10 cm, glass support

• Synonym(e): Silica F₂₅₄ Chromatography Plates
• UNSPSC-Code: 41115711
• NACRES: NB.21

Eigenschaften

• Materialien: glass support; silica gel 60 matrix
• Qualitätsniveau: 100
• Leistungsmerkmale: binder Organic Polymer; fluorescent indicator
• Verpackung: pkg of 25 plates
• Methode(n): thin layer chromatography (TLC): suitable
• Schichtdicke: 200 μm
• Platte L × B: 5 cm × 10 cm
• Partikelgröße: 5-6 μm
• Porengröße: 60 Å medium pore diameter
• Lagertemp.: 2-30°C

Beschreibung

Allgemeine Beschreibung

25 Glass plates 5 x 10 cm

HPTLC plates deliver fast and quantitative analysis of complex samples for manual or automated use. Our HPTLC silica plates work three times faster than conventional TLC plates – and they are more sensitive. The particle sizes range in HPTLC is between 4-8 µm, which creates a smoother surface and a higher separation power than TLC plates.

Anwendung

• Stability indicating high performance thin layer chromatography method development and validation for quantitative determination of tetracycline hydrochloride: In this study, the use of Silica gel F₂₅₄ HPTLC plates is optimized for analyzing tetracycline hydrochloride in pharmaceutical forms. This method serves as a stability-indicating test, crucial for ensuring the safety and efficacy of this common antibiotic, highlighting the material′s importance in regulatory compliance and drug development (Gashaw et al., 2024).


• Robust high-performance thin-layer chromatography (HPTLC) method for stability assessment and simultaneous quantification of epigallocatechin-3-gallate and rosmarinic acid in lipid-based nanoparticles and biological matrices: This research uses Silica gel F₂₅₄ HPTLC plates to develop a high-performance chromatographic method for assessing the stability and concentration of bioactive compounds in complex samples. The technique is essential for advanced material science and biomedical research, providing accurate and reproducible results (Koli et al., 2024).

Verlinkung

Replaces: 105616-2; 105616

Hinweis zur Analyse

Spezifische Oberfläche (nach BET; 5-Pkt. Messung): 480 - 540 m²/g
Porenvolumen (N₂-Isotherme): 0.74 - 0.84 ml/g
d 50 (Laserbeugung, Kornanalyse): 5 - 7 µm
Schichtdicke: 150 - 200 µm
Dickenschwankung: ≤ 35 µm
Anwendungstechnische Prüfungen:
A) Farbstofftest
hRf-Werte
- Bleu VIF Organol, Farbstofftest, lipophil:
17 - 22
- Ceresschwarz G, Farbstofftest, lipophil: 38 - 43
- Ceresviolett BRN, Farbstofftest, lipophil: 58 - 64
Trennzahl
- Farbstofftest, lipophil:
≥ 10.0
B) Steriodtest
hRf-Werte
- Hydrocortison,Steroidtest:
23 - 29
- Reichstein S, Steroidtest: 36 - 45
- Methyltestosteron, Steroidtest: 43 - 52
Trennzahl
- Steroidtest:
≥ 10.5
Typische Werte, gemessen an einer konditionierten Platte
Eluent A) Toluol (45% rel.Feuchte)
Eluent B) Ethylacetat/Toluol (95+5 v/v; 20% rel.Feuchte)