05-886R

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 94-10C-9-10C-2, rabbit monoclonal

clone 94-10C-9-10C-2, from rabbit

• Synonym(e): signal transducer and activator of transcription 5A/B
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 94-10C-9-10C-2, monoclonal
• Speziesreaktivität: mouse, human, rat
• Methode(n): western blot: suitable
• Isotyp: IgG
• NCBI-Hinterlegungsnummer: NP_003143.2
• UniProt-Hinterlegungsnummer: P42229
• Versandbedingung: dry ice
• Posttranslationale Modifikation Target: phosphorylation (pTyr694/pTyr699)
• Angaben zum Gen: human ... STAT5A(6776)

Beschreibung

Allgemeine Beschreibung

STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases (1,2). In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT (1). Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1). After dimerization, the STATs accumulate in the nucleus by way of nuclear transport (1). STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production (1,2,3). The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.

Spezifität

Recognizes phosphorylated STAT5A/B atTyr694/Tyr699.

Immunogen

Epitope: Tyr694/Tyr699

KLH-conjugated linear peptide corresponding to phosphorylated human STAT5A/B at and around Tyr694/Tyr699.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Transkriptionsfaktoren

Transkriptionsfaktoren

Use Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 94-10C-9-10C-2 (rabbit monoclonal antibody) validated in WB to detect phospho-STAT5A/B (Tyr694/699) also known as signal transducer & activator of transcription 5A/B.

Qualität

Evaluated by Western Blot in U937 cell lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected STAT5 A/B on 10 µg of U937 cell lysate.

Zielbeschreibung

95 kDa

Physikalische Form

Protein A purified

Format: Purified

Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 30% Glycerol.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Hinweis zur Analyse

Control
GMCSF treated and untreated U937 cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable