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05-669

Sigma-Aldrich

Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6

clone 11E6, Upstate®, from mouse

Synonym(e):

Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

11E6, monoclonal

Speziesreaktivität

rat, canine, mouse, human

Hersteller/Markenname

Upstate®

Methode(n)

ELISA: suitable
western blot: suitable

Isotyp

IgG1κ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

phosphorylation (pSer473)

Angaben zum Gen

dog ... Akt1(490878)
human ... AKT1(207)

Allgemeine Beschreibung

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB-β or RacPK-β) and Akt3 (also designated PKB-γ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the 85 kDa subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1- stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin. Taken together, this data strongly suggests that the protein signals downstream of the PI kinases.

Spezifität

Recognizes phosphorylated Akt1/PKBα at Ser473, Mr 60 kDa. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.

Anwendung

ELISA:
A previous lot was recommended at 0.05 mg/mL by an independent laboratory for ELISA.
This Anti-Akt1 Antibody, clone 11E6 is validated for use in ELISA, WB for the detection of phospho-Akt1/PKBα (Ser473).

Qualität

Evaluated by western blot on lysates from mouse NIH-3T3 fibroblasts treated with PDGF.

Western Blot Analysis: 0.5-2 µg/mL of this antibody detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 100 ng/mL PDGF for 20 minutes.

Zielbeschreibung

60 kDa

Physikalische Form

Format: Purified
Purified mouse monoclonal IgG1κ lyophilized from 1 mL 2X PBS, PEG, sucrose, and 0.09% sodium azide.
Reconstitute with 1 mL H2O (15 min. at RT). Concentration after reconstitution: 100 µg/mL

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt.
Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 2-8°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
PDGF treated NIH-3T3 cell lysates.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Piktogramme

Skull and crossbones

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Lagerklassenschlüssel

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Neuroprotective effects of activated protein C through induction of insulin-like growth factor-1 (IGF-1), IGF-1 receptor, and its downstream signal phosphorylated serine-threonine kinase after spinal cord ischemia in rabbits.
Yamauchi, T; Sakurai, M; Abe, K; Takano, H; Sawa, Y
Stroke null
Akt1 deficiency modulates reward learning and reward prediction error in mice.
Y-C Chen,Y-W Chen,Y-F Hsu,W-T Chang,C K Hsiao,M-Y Min,W-S Lai
Genes, Brain, and Behavior null
P21 (waf1/cip1) is required for non-small cell lung cancer sensitive to Gefitinib treatment.
Zhao YF, Wang CR, Wu YM, Ma SL, Ji Y, Lu YJ
Biomedicine and Pharmacotherapy null
Yoshikazu Matsuoka et al.
Stem cells (Dayton, Ohio), 29(11), 1783-1791 (2011-09-08)
Although c-kit is expressed highly on murine hematopoietic stem cells (HSCs) and essential for bone marrow (BM) hematopoiesis, the significance of the high level of expression of c-kit on HSCs was not well determined. We show here that CD150(+) CD48(-)
Activated protein C has a protective effect against myocardial I/R injury by improvement of endothelial function and activation of AKT1.
Maehata, Y; Miyagawa, S; Sawa, Y
Testing null

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