930423

CP-alkyne

≥95%

• Synonym(e): 2,4,6-Trimethyl-1-(methyl((pent-4-yn-1-yloxy)carbonyl)amino)pyridin-1-ium tetrafluoroborate
• Empirische Formel (Hill-System): C₁₅H₂₁BF₄N₂O₂
• Molekulargewicht: 348.14
• UNSPSC-Code: 41116164
• NACRES: NA.22

Eigenschaften

• Beschreibung: Application: Chemoproteomics
• Qualitätsniveau: 100
• Assay: ≥95%
• Form: liquid
• Lagertemp.: −20°C
• SMILES String: CC1=CC(C)=[N+](N(C(OCCCC#C)=O)C)C(C)=C1.F[B-](F)(F)F

Beschreibung

Anwendung

CP-alkyne is a probe that can be used to photochemically label tryptophans. A method was developed using cysteine-reactive compounds including this one to allow for unbiased analysis of proteomic data in quantitative applications (Zanon et al. 2021). The method uses light or heavy labelling with the isotopically labelled desthiobiotin azide (isoDTB) tag for mass spectrometry analysis (Zanon et al. 2020). Analysis then uses the isotopic tandem orthogonal proteolysis activity-based protein profiling (isoTOP-ABPP) workflow (Weerapana et al. 2010, Backus et al. 2016)

Sonstige Hinweise

1. Profiling the proteome-wide selectivity of diverse electrophiles

2. A quantitative thiol reactivity profiling platform to analyze redox and electrophile reactive cysteine proteomes

3. Ethynylation of Cysteine Residues: From Peptides to Proteins in Vitro and in Living Cells

4. A Chemoproteomic Platform To Assess Bioactivation Potential of Drugs

5. Inhibition of Zinc-Dependent Histone Deacetylases with a Chemically Triggered Electrophile

6. Reversibility of Covalent Electrophile-Protein Adducts and Chemical Toxicity

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable