ZRB1018

Anti-LEAP2 Antibody, clone 1D17 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Liver-expressed antimicrobial peptide 2
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1D17, recombinant monoclonal
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 8.81 kDa; observed mol wt ~10 kDa
• Produit purifié par: using Protein A
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rat, mouse
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: Internal
• Numéro d'accès Protein ID: NP_443203
• Numéro d'accès UniProt: Q969E1
• Autre catégorie plus écologique: Aligned,
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... LEAP2(116842)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 1D17 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects LEAP2. It targets an epitope within 14 amino acids from the internal region.

Immunogène

KLH-conjugated linear peptide corresponding to 14 amino acids from the internal region of human LEAP2.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Human LEAP2.

Western Blotting Analysis (WB): 1:1,000 dilution of this antibody detected recombinant Human LEAP2.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LEAP2 in human skeletal muscle.

Affinity Binding Assay: A representative lot of this antibody bound LEAP2 peptide with a KD of 7.0 x 10-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected LEAP2 in human skeletal muscle tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Description de la cible

Liver-expressed antimicrobial peptide 2 (UniProt: Q969E1; also known as LEAP-2) is encoded by the LEAP2 gene (Gene ID: 116842) in human. Cationic antimicrobial peptides are widely distributed in nature and have a key role in innate immunity and host defense and induce the direct destruction of microorganisms. LEAP2 is a 40-residue cationic antimicrobial peptide that is highly conserved in mammals. LEAP-2 is mainly expressed in the liver but is also produced in smaller quantities in other tissues. It is synthesized with a signal peptide (aa 1-22) and a propeptide (aa 23-37) that are subsequently cleaved off to generate the active mature peptide (aa 38-77). Its primary structure and disulfide bond pattern does not exhibit any similarity to any known peptide. It has a compact central core with disorder at the N- and C-termini. The core comprises a β-hairpin and a 3(10)- helix that are braced by disulfide bonds between Cys17-28 and Cys23-33 (in mature peptide) and is further stabilized by a network of hydrogen bonds. Its expression appears to be well regulated at the transcriptional level. LEAP2 gene consists of three exons and two introns. LEAP2 exhibits dose-dependent antimicrobial activity with an IC50 of about 5 μM against Saccharomyces cerevisiae and Bacillus subtilis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Henriques, ST., et al. (2010). Chembiochem. 11(15); 148-157; Krause, A., et al. (2003). Protein Sci. 12(1); 143-152).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable