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Millipore

Mueller Hinton Broth

suitable for microbiology, NutriSelect® Plus

Synonyme(s) :

MH Broth; Antibacterial potency testing media; Antimicrobial susceptibility testing media

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About This Item

Code UNSPSC :
41171504
Nomenclature NACRES :
NA.74

Stérilité

non-sterile

Niveau de qualité

Gamme de produits

BioChemika

Forme

powder

Durée de conservation

limited shelf life, expiry date on the label

Composition

beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L

Fabricant/nom de marque

NutriSelect® Plus

Conditions de stockage

protect from light

Technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

pH final

7.4±0.2 (25 °C)

Application(s)

clinical testing
food and beverages

microbiology

Température de stockage

15-25°C

Adéquation

nonselective for bacteria (General Media)

Description générale

Mueller Hinton Broth is used for determining minimal inhibitory concentrations (MICs). Mueller Hinton medium is recommended by FDA, World Health Organization, and NCCLS for testing the most encountered aerobic and facultative anaerobic bacteria in food and clinical material. The medium shows good batch-to-batch reproducibility, it is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens.

Application

Mueller Hinton broth is primarily used to determine the MIC (minimum inhibitory concentration) for several antimicrobial agents. It can also be used to cultivate fastidious organisms like Meningococcus and Gonococcus

Notes préparatoires

Dissolve 21 g in 1 litre of distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Branko Jovčić et al.
Antibiotics (Basel, Switzerland), 9(12) (2020-12-17)
The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven
Konrad Gwozdzinski et al.
Journal of clinical microbiology, 56(5) (2018-03-09)
The plasmid-located colistin resistance gene mcr-1 confers low-level resistance to colistin, a last-line antibiotic against multidrug-resistant Gram-negative bacteria. Current CLSI-EUCAST recommendations require the use of a broth microdilution (BMD) method with cation-adjusted Mueller-Hinton (CA-MH) medium for colistin susceptibility testing, but
Pin-Kuang Lai et al.
Biotechnology and bioengineering, 116(10), 2439-2450 (2019-06-19)
Proline-rich antimicrobial peptides (PrAMPs) kill bacteria via a nonlytic mechanism in which they permeate through the outer membrane, utilize protein-mediated transport across the inner membrane, and target the ribosome to inhibit protein synthesis. We previously reported that substitutions of oncocin
J.H. Muller et al.
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 48, 330-330 (1941)
Tracy Lackraj et al.
Journal of bacteriology, 202(11) (2020-04-02)
Dps, a DNA-binding protein from starved cells in Escherichia coli, is part of the bacterial defense system that protects DNA against various cellular stresses. Our lab previously demonstrated that a novel antimicrobial peptide, WRWYCR, enhances acid-induced killing of enterohemorrhagic Escherichia

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