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Key Documents

SRP6057

Sigma-Aldrich

Activin A from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonyme(s) :

FRP, FSH-releasing protein, Inhibin beta-1

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

mouse

Produit recombinant

expressed in E. coli

Pureté

≥95% (HPLC)
≥95% (SDS-PAGE)

Forme

lyophilized

Poids mol.

26.2 kDa

Conditionnement

pkg of 10 μg

Technique(s)

cell culture | embryo: suitable

Numéro d'accès NCBI

P08476

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

mouse ... INHBA(3624)

Description générale

Activin A is a member of the TGF (transforming growth factor)-β family of proteins produced by many cell types throughout development. Mouse Activin A has 100% amino acid sequence identity to human, rat, porcine, bovine and feline proteins. Recombinant mouse Activin A is a nonglycosylated homodimer, containing two 117 amino acid chains, with a total molecular weight of 26.2 kDa.

Application

Activin A has been used:
  • to induce differentiation of embryonic stem cells to pancreatic β cells
  • in the induction of bone marrow-derived mesenchymal stem cells to insulin-producing cells
  • to induce endoderm differentiation from human embryonic stem cells
  • as a component of β cell induction medium.

Actions biochimiques/physiologiques

Activins interact with Type I and Type II serine/threonine kinases to signal to SMAD proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, metabolism, etc. Activin A is a homodimer of two β A chains and is not biologically active until the N terminal propeptide is cleaved from each chain. Activin A is involved in mesoderm formation and hematopoietic development.

Forme physique

Recombinant mouse Activin A is lyophilized with 0.02% TFA.

Notes préparatoires

Centrifuge the vial prior to opening.

Reconstitution

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water, which can then be further diluted to other aqueous solutions.

Autres remarques

MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Inducing embryonic stem cells to differentiate into pancreatic beta cells by a novel three-step approach with activin A and all-trans retinoic acid.
Shi Y
Stem Cells, 23, 656-662 (2005)
Functional analysis of activins during mammalian development.
Matzuk MM
Nature, 374, 354-356 (1995)
Different phenotypes for mice deficient in either activins or activin receptor type II.
Matzuk MM
Nature, 374, 356-360 (1995)
In vitro derivation of functional insulin-producing cells from human embryonic stem cells.
Jiang W
Cell Research, 17, 333-344 (2007)
Requirement for activin A and transforming growth factor--beta 1 pro-regions in homodimer assembly.
Gray AM
Science, 247, 1328-1330 (1990)
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